Crude glycerol as substrate for oleaginous yeasts 作为含油酵母基板的粗甘油

Diverse oleaginous yeasts can grow and accumulate lipid on a broad range of competitive substrates. In this study crude glycerol from conventional biodiesel production was used as the substrate for lipid production via oleaginous yeasts. Various cultivation conditions were tested – different C/N ratios, temperatures and addition of salts. Candida lipolytica, Yarrowia lipolytica, Trichosporon fermentans produced up to maximum of 22 % (w/w) lipid relating to the biomass at specific cultivation conditions. Scale up experiments revealed the further challenges of the lipid production via oleaginous yeasts adhesion of the yeast, production of citric acid, low biomass and lipid yield. The lipid yield was 17 % (w/w) relating to the biomass despite addition of detergent.